Phytochemical Composition and Antioxidant Activity of Methanol, Ethanol, and Water Extracts from Pruned Harumanis Mango Leaves
DOI:
https://doi.org/10.36877/aafrj.a0000553Abstract
This study investigates the phytochemical composition, antioxidant activity, total phenolic content, and total flavonoid content of methanol, ethanol, and water extracts from pruned Harumanis mango leaves. High-performance liquid chromatography (HPLC) detected mangiferin, with all extracts yielding positive results. The detected phytochemicals include flavonoids, cardiac glycosides, tannins, and steroids. Among the solvents tested, ethanol and methanol were identified as the most effective for extracting phytochemicals from Harumanis mango leaves. Both extracts contained flavonoids, cardiac glycosides, tannins, and steroids. The ethanol extract exhibited the highest antioxidant activity (94.559 ± 0.425%), total phenolic content (0.055 ± 0.002 GAE mg/ml), and total flavonoid content (0.071 ± 0.002 mg/ml). Ethanol was the optimal solvent for extracting bioactive compounds from Harumanis pruned leaves, likely due to its less polar nature, which enhances extraction efficiency. The study concludes that ethanol is the most effective solvent for extracting bioactive compounds, providing the highest antioxidant activity, phenolic, and flavonoid content. This research offers valuable insights into the potential health benefits of Harumanis pruned leaves, especially concerning their antioxidant properties attributed to specific phytochemicals.
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