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Decoding the mystery of how bacteria “talk”: Among Gram-negative microorganisms

Wei-Sin Tan, Jodi Woan-Fei Law, Vengadesh Letchumanan, Kok-Gan Chan Abstract - 525 PDF - 19


To date, microbial diversity is still the least well understood component of biodiversity. Bacteria are the most abundant microorganisms where most species are often found ubiquitous. Microorganisms such as bacteria are diverse in their impacts such as in spreading of infectious diseases or play a valuable role in biotechnological purposes. Hence, it is interesting to gain a look upon the ways where bacteria regulate their daily processes in the environment. Bacteria communicate with each other through extracellular signalling molecules or also known as autoinducers (AIs) that are produced, detected and show response. This process is termed as quorum sensing (QS) which indicates that bacteria do communicate in order to perform various physiological activities. QS enable bacteria to have the advantages that are unattainable as individual bacterial cell. This review emphases on the characteristics of quorum sensing (QS) and its benefits in understanding different kind of bacterial QS-dependent activities. This fundamental insight from QS system will enable us to manage bacterial activities by targeting their communication circuit.

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Letchumanan, V, Chan, K-G, and Lee, L-H. Vibrio parahaemolyticus: A review on the pathogenesis, prevalence, and advance molecular identification techniques. Front Microbiol, 2014; 5: 705.

Letchumanan, V, Yin, W-F, Lee, L-H, et al. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in Malaysia. Front Microbiol, 2015; 6: 33.

Letchumanan, V, Pusparajah, P, Tan, LT-H, et al. Occurrence and antibiotic resistance of Vibrio parahaemolyticus from shellfish in Selangor, Malaysia. Front Microbiol, 2015; 6: 1417.

Heng, S-P, Letchumanan, V, Deng, C-Y, et al. Vibrio vulnificus: An environmental and clinical burden. Front Microbiol, 2017; 8: 997.

Letchumanan, V, Wong, P-C, Goh, B-H, et al. A review on the characteristics, taxanomy and prevalence of Listeria monocytogenes. Prog

Microbes Mol Biol, 2018; 1(1).

Letchumanan, V, Ab Mutalib, N-S, Wong, SH, et al. Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia. Prog Microbes Mol Biol, 2019; 1(1).

Letchumanan, V, Loo, K-Y, Law, JW-F, et al. Vibrio parahaemolyticus: The protagonist of foodborne diseases. Prog Microbes Mol Biol, 2019; 1(1).

Lee, L-H, Ab Mutalib, N-S, Law, JW-F, et al. Discovery on antibiotic resistance patterns of Vibrio parahaemolyticus in Selangor reveals carbapenemase producing Vibrio parahaemolyticus in marine and freshwater fish. Front Microbiol, 2018; 9: 2513.

Kaufmann, GF, Sartorio, R, Lee, S-H, et al. Revisiting quorum sensing: Discovery of additional chemical and biological functions for 3-oxo-N acylhomoserine lactones. Proc Natl Acad Sci USA, 2005; 102(2): 309–314.

Moré, MI, Finger, LD, Stryker, JL, et al. Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates. Science, 1996; 272(5268): 1655–1658.

Fuqua, C, Winans, SC, and Greenberg, EP. Census and consensus in bacterial ecosystems: The LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu Rev Microbiol, 1996; 50(1): 727–751.

Swift, S, Winson, M, Chan, P, et al. A novel strategy for the isolation of luxl homologues: evidence for the widespread distribution of a LuxR: Luxl superfamily in enteric bacteria. Mol Microbiol, 1993; 10(3): 511–520.

Williams, P. Quorum sensing, communication and cross-kingdom signalling in the bacterial world. Microbiol, 2007; 153(12): 3923–3938.

Bassler, BL and Losick, R. Bacterially speaking. Cell, 2006; 125(2): 237–246.

Eberhard, A. Inhibition and activation of bacterial luciferase synthesis. J Bacteriol, 1972; 109(3): 1101–1105.

Nealson, KH, Platt, T, and Hastings, JW. Cellular control of the synthesis and activity of the bacterial luminescent system. J Bacteriol, 1970; 104(1): 313–322.

Ruby, EG and Lee, K-H. The Vibrio fischeri-Euprymna scolopes light organ association: current ecological paradigms. Appl Environ Microbiol, 1998; 64(3): 805–812.

De Kievit, T. Quorum sensing in Pseudomonas aeruginosa biofilms. Environ Microbiol, 2009; 11(2): 279–288.

Suppiger, A, Schmid, N, Aguilar, C, et al. Two quorum sensing systems control biofilm formation and virulence in members of the Burkholderia cepacia complex. Virulence, 2013; 4(5): 400–409.

Cámara, M, Hardman, A, Williams, P, et al. Quorum sensing in Vibrio cholerae. Nat Genet, 2002; 32(2): 217–218.

Senadheera, D and Cvitkovitch, DG. Quorum sensing and biofilm formation by Streptococcus mutans. In: Bacterial signal transduction: Networks and drug targets. New York: Springer; 2008: 178–188.

Carter, GP, Purdy, D, Williams, P, et al. Quorum sensing in Clostridium difficile: Analysis of a luxS-type signalling system. J Med Microbiol, 2005; 54(2): 119–127.

Andersson, RA, Eriksson, AR, Heikinheimo, R, et al. Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: The role of expR(Ecc). Mol Plant-Microbe Interact, 2000; 13(4): 384–393.

Tan, W-S, Khan, S, Chew, H, et al. Unusual multiple production of N acylhomoserine lactones a by Burkholderia sp. strain C10B isolated from dentine caries. Sensors, 2014; 14(5): 8940–8949.

Deng, Y, Lim, A, Lee, J, et al. Diffusible signal factor (DSF) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens. BMC Microbiol, 2014; 14(1): 14–51.

Dong, Y-H and Zhang, L-H. Quorum sensing and quorum-quenching enzymes. J Microbiol, 2005; 43(1): 101–109.

Kim, K, Kim, YU, Koh, BH, et al. HHQ and PQS, two Pseudomonas aeruginosa quorum‐sensing molecules, down‐regulate the innate immune responses through the nuclear factor‐κB pathway. Immunology, 2010; 129(4): 578–588.

Olson, ME, Todd, DA, Schaeffer, CR, et al. Staphylococcus epidermidis agr quorum-sensing system: Signal identification, cross talk, and importance in colonization. J Bacteriol, 2014; 196(19): 3482–3493.

Kher, H-L, Krishnan, T, Letchumanan, V, et al. Characterization of quorum sensing genes and N-acyl homoserine lactones in Citrobacter amalonaticus strain YG6. Gene, 2019; 684: 58–69.

Joint, I. Bacterial conversations: Talking, listening and eavesdropping. A NERC Discussion Meeting held at the Royal Society on 7 December 2005. J Royal Soc Interface, 2006; 3(8): 459–463.

Niu, C, Clemmer, KM, Bonomo, RA, et al. Isolation and characterization of an autoinducer synthase from Acinetobacter baumannii. J Bacteriol, 2008; 190(9): 3386–3392.

Jahid, IK, Lee, N-Y, Kim, A, et al. Influence of glucose concentrations on biofilm formation, motility, exoprotease production, and quorum sensing in Aeromonas hydrophila. J Food Prot, 2013; 76(2): 239–247.

Swift, S, Karlyshev, AV, Fish, L, et al. Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: Identification of the LuxRI homologs AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J Bacteriol, 1997; 179(17): 5271–5281.

Wang, C, Yan, C, Fuqua, C, et al. Identification and characterization of a second quorum-sensing system in Agrobacterium tumefaciens A6. J Bacteriol, 2014; 196(7): 1403–1411.

Riedel, K, Hentzer, M, Geisenberger, O, et al. N-acylhomoserine lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms. Microbiol, 2001; 147(12): 3249–3262.

Nickzad, A, Lépine, F, and Déziel, E. Quorum sensing controls swarming motility of Burkholderia glumae through regulation of rhamnolipids. PLoS One, 2015; 10(6): e0128509.

Ulrich, RL, DeShazer, D, Brueggemann, EE, et al. Role of quorum sensing in the pathogenicity of Burkholderia pseudomallei. J Med Microbiol, 2004; 53(11): 1053–1064.

McClean, KH, Winson, MK, Fish, L, et al. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiol, 1997; 143(12): 3703–3711.

Chalupowicz, L, Barash, I, Panijel, M, et al. Regulatory interactions between quorum-sensing, auxin, cytokinin, and the Hrp regulon in relation to gall formation and epiphytic fitness of Pantoea agglomerans pv. gypsophilae. Mol Plant-Microbe Interact, 2009; 22(7): 849–856.

Joe, MM, Benson, A, Saravanan, V, et al. In vitro antibacterial activity of nanoemulsion formulation on biofilm, AHL production, hydrolytic enzyme activity, and pathogenicity of Pectobacterium carotovorum sub sp. carotovorum. Physiol Mol Plant Pathol, 2015; 91: 46–55.

Burton, E, Read, H, Pellitteri, M, et al. Identification of acylhomoserine lactone signal molecules produced by Nitrosomonas europaea strain Schmidt. Appl Environ Microbiol, 2005; 71(8): 4906–4909.

Chug, R, Khosla, B, and Singh, M. Modulation of the extracellular polymeric substances (EPS) production by quorum sensing (QS) in bacteria. Int J Curr Microbiol Appl Sci, 2015; 4(6): 884–896.

Jakobsen, TH, Bjarnsholt, T, Jensen, PØ, et al. Targeting quorum sensing in Pseudomonas aeruginosa biofilms: Current and emerging inhibitors. Future Microbiology, 2013; 8(7): 901–921.

Jimenez, PN, Koch, G, Thompson, JA, et al. The multiple signaling systems regulating virulence in Pseudomonas aeruginosa. Microbiol Mol Biol Rev, 2012; 76(1): 46–65.

Lithgow, JK, Wilkinson, A, Hardman, A, et al. The regulatory locus cinRI in Rhizobium leguminosarum controls a network of quorumsensing loci. Mol Microbiol, 2000; 37(1): 81–97.

Horng, YT, Deng, SC, Daykin, M, et al. The LuxR family protein SpnR functions as a negative regulator of N‐acylhomoserine lactone‐dependent quorum sensing in Serratia marcescens. Mol Microbiol, 2002; 45(6): 1655–1671.

Rice, S, Koh, K, Queck, S, et al. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. J Bacteriol, 2005; 187(10): 3477–3485.

Cao, J-G and Meighen, E. Purification and structural identification of an autoinducer for the luminescence system of Vibrio harveyi. J Biol Chem, 1989; 264(36): 21670–21676.

Manefield, M, Harris, L, Rice, SA, et al. Inhibition of luminescence and virulence in the black tiger prawn (Penaeus monodon) pathogen Vibrio harveyi by intercellular signal antagonists. Appl Environ Microbiol, 2000; 66(5): 2079–2084.

Atkinson, S, Chang, CY, Patrick, HL, et al. Functional interplay between the Yersinia pseudotuberculosis YpsRI and YtbRI quorum sensing systems modulates swimming motility by controlling expression of flhDC and fliA. Mol Microbiol, 2008; 69(1): 137–151.

Chan, K-G, Cheng, HJ, Chen, JW, et al. Tandem mass spectrometry detection of quorum sensing activity in multidrug resistant clinical isolate Acinetobacter baumannii. Sci World J, 2014; 2014: 1–6.

Churchill, ME, Sibhatu, HM, and Uhlson, CL. Defining the structure and function of acyl-homoserine lactone autoinducers. Methods Mol Biol, 2011; 692: 159–171.

Fuqua, C and Greenberg, EP. Signalling: Listening in on bacteria: acyl-homoserine lactone signalling. Nat Rev Mol Cell Biol, 2002; 3(9): 685–695.

Kaplan, HB and Greenberg, E. Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. J Bacteriol, 1985; 163(3): 1210–1214.

Boyer, M and Wisniewski-Dye, F. Cell–cell signalling in bacteria: not simply a matter of quorum. FEMS Microbiol Ecol, 2009; 70(1): 1–19.

Evans, K, Passador, L, Srikumar, R, et al. Influence of the MexABOprM multidrug efflux system on quorum sensing in Pseudomonas aeruginosa. J Bacteriol, 1998; 180(20): 5443–5447.

Pearson, JP, Feldman, M, Iglewski, BH, et al. Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection. Infect Immun, 2000; 68(7): 4331–4334.

Myszka, K and Czaczyk, K. N-Acylhomoserine Lactones (AHLs) as phenotype control factors produced by Gram-negative bacteria in natural ecosystems. Polish J Environ Stud, 2012; 21(1): 15–21.

Schaefer, AL, Val, DL, Hanzelka, BL, et al. Generation of cell-tocell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. Proc Nat Acad Sci USA, 1996; 93(18): 9505.

Schauder, S and Bassler, BL. The languages of bacteria. Genes Dev, 2001; 15(12): 1468–1480.

Choi, S and Greenberg, E. The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene activating domain. Proc Nat Acad Sci, 1991; 88(24): 11115–11119.

Slock, J, VanRiet, D, Kolibachuk, D, et al. Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis. J Bacteriol, 1990; 172(7): 3974–3979.

Ng, W-L and Bassler, BL. Bacterial quorum-sensing network architectures. Annu Rev Genet, 2009; 43: 197–222.

Flavier, AB, Ganova-Raeva, LM, Schell, MA, et al. Hierarchical autoinduction in Ralstonia solanacearum: control of acyl-homo-serine lactone production by a novel autoregulatory system responsive to 3 hydroxypalmitic acid methyl ester. J Bacteriol, 1997; 179(22): 7089–7097.

Pearson, JP, Pesci, EC, and Iglewski, BH. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol, 1997; 179(18): 5756–5767.

Chugani, S and Greenberg, EP. An evolving perspective on the Pseudomonas aeruginosa orphan quorum sensing regulator QscR. Front Cell Infect Microbiol, 2014; 4: 152.

Steindler, L and Venturi, V. Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol Lett, 2007; 266(1): 1–9.

Winson, MK, Swift, S, Fish, L, et al. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiol Lett, 1998; 163(2): 185–192.

Pearson, JP, Gray, KM, Passador, L, et al. Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci USA, 1994; 91(1): 197–201.

Dong, YH, Zhang, XF, Soo, HML, et al. The two‐component response regulator PprB modulates quorum‐sensing signal production and global gene expression in Pseudomonas aeruginosa. Mol Microbiol, 2005; 56(5): 1287–1301.

Khan, SR, Mavrodi, DV, Jog, GJ, et al. Activation of the phz operon of Pseudomonas fluorescens 2-79 requires the LuxR homolog PhzR, N-(3 OH-hexanoyl)-L-homoserine lactone produced by the LuxI homolog PhzI, and a cis-acting phz box. J Bacteriol, 2005; 187(18): 6517–6527.

Sifri, CD. Quorum sensing: Bacteria talk sense. Clin Infect Dis, 2008; 47(8): 1070–1076.

Lee, J and Zhang, L. The hierarchy quorum sensing network in Pseudomonas aeruginosa. Protein & Cell, 2015; 6(1): 26–41.

Deng, Y, Wu, Je, Tao, F, et al. Listening to a new language: DSF based quorum sensing in Gram-negative bacteria. Chemical Reviews, 2010; 111(1): 160–173.

Ionescu, M, Baccari, C, Da Silva, AM, et al. Diffusible signal factor (DSF) synthase RpfF of Xylella fastidiosa is a multifunction protein also required for response to DSF. J Bacteriol, 2013; 195(23): 5273–5284.

Ryan, RP, An, S-q, Allan, JH, et al. The DSF family of cell–cell signals: An expanding class of bacterial virulence regulators. PLoS Path, 2015; 11(7): e1004986.

Bogino, PC, Nievas, FL, and Giordano, W. A review: Quorum sensing in Bradyrhizobium. Applied Soil Ecology, 2015; 94: 49–58.

Kai, K, Ohnishi, H, Shimatani, M, et al. Methyl 3‐hydroxymyristate, a diffusible signal mediating phc quorum sensing in Ralstonia solanacearum. ChemBioChem, 2015; 16(16): 2309–2318.

Queck, SY, Jameson-Lee, M, Villaruz, AE, et al. RNAIII-independent target gene control by the agr quorum-sensing system: Insight into the evolution of virulence regulation in Staphylococcus aureus. Mol Cell, 2008; 32(1): 150–158.

Pestova, E, Håvarstein, L, and Morrison, D. Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto induced peptide pheromone and a two‐component regulatory system. Mol Microbiol, 1996; 21(4): 853–862.

Nakano, MM, Xia, L, and Zuber, P. Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis. J Bacteriol, 1991; 173(17): 5487–5493.

Del Papa, MF and Perego, M. Enterococcus faecalis virulence regulator FsrA binding to target promoters. J Bacteriol, 2011; 193(7): 1527–1532.

Law JW-F, Ab Mutalib N-S, Chan K-G, et al. An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food. Front Microbiol. 2015; 6:1227.

Law JW-F, Ab Mutalib N-S, Chan K-G et al. Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. Front Microbiol. 2015; 5:770.

Letchumanan V, Ser H-L, Tan W-S, et al. Genome sequence of Vibrio parahaemolyticus VP152 strain isolated from Penaeus indicus in Malaysia. Front Microbiol. 2016; 7:1410.

Letchumanan V, Ser H-L, Chan K-G, et al. Genome sequence of Vibrio parahaemolyticus VP103 strain isolated from shrimp in Malaysia. Front Microbiol. 2016; 7:1496.

Letchumanan V, Chan K-G, Khan TM, et al. Bile sensing: The activation of Vibrio parahaemolyticus virulence. Front Microbiol. 2017; 8:728.

Letchumanan V, Chan K-G, Lee L-H. An insight of traditional plasmid curing in Vibrio species. Front Microbiol. 2015; 6:735.

Law JWF, Letchumanan V, Chan KG, et al. Insights into detection and identification of foodborne pathogens. Food Borne Pathogens and Antibiotic Resistance. 2016.

Vengadesh L, Son R, Yoke-Kqueen C. Vibrio cholerae from different seafood obtained from wetmarket and supermarket. Inter Food Res J. 2012; 19(1).

Lee L-H and Raghunath P. Vibrionaceae diversity, multidrug resistance and management. Front Microbiol. 2018; 9:563.

Eng S-K, Pusparajah P, Ab Mutalib N-S, et al. Salmonella: a review on pathogenesis, epidemiology and antibiotic resistance. Front Life Sci. 2015; 8(3):284-93.

Khoo C-H, Cheah Y-K, Lee L-H, et al. Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR. Antonie van Leeuwenhoek. 2009; 96(4):441.

Cheah Y-K, Salleh NA, Lee L-H, et al. Comparison of PCR fingerprinting techniques for the discrimination of Salmonella enterica subsp. enterica serovar Weltevreden isolated from indigenous vegetables in Malaysia. World J Microbiol Biotechnol. 2008; 24(3):327.

Yoke-Kqueen C, Learn‐Han L, Noorzaleha A, et al. Characterization of multiple antimicrobial resistant Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry in Malaysia. Lett Appl Microbiol. 2008; 46(3):318-24.

Learn-Han L, Yoke-Kqueen C, Salleh NA, et al. Analysis of Salmonella Agona and Salmonella Weltevreden in Malaysia by PCR fingerprinting and antibiotic resistance profiling. Antonie Van Leeuwenhoek. 2008; 94(3):377.


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