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Characterization of fatty acid based nanostructured lipid carrier (NLC) and their sustained release properties

Han-Choi Yew, Misni Misran Abstract - 239 PDF - 171


Nanostructured lipid carrier (NLC) is a second generation lipid nanoparticle formed by blends of solid and liquid lipids through hot homogenization technique. In this study, arachidic acid (C20) and erucic acid (C22:1) were used as solid lipids while oleic acid as liquid lipid in the preparation of NLC. Five types of NLC were prepared by varying the amount of oleic acid (C18:1) with respect to arachidic acid while maintaining the amount of erucic acid. Physical stability of the prepared NLC was characterized by its size and zeta potential for a period of 28 days. The results showed that the size of NLCs were between 200 to 260 nm with zeta potential of -55 to -40 mV. Differential scanning calorimetry (DSC) data showed that presence of oleic acid reduces the crystallinity of nanoparticle. Apart from that, depending on the compositions, the morphology of NLC examined under transmission electron microscopy’s (TEM) was round to elongate in shape. Then, active ingredients of ascorbic acid, caffeine and lidocaine with varied hydrophilicity were then loaded into the NLC. Lidocaine has an encapsulation efficiency of nearly 78% while caffeine reaches 43%. Surprisingly, ascorbic acid which is hydrophilic gave a comparative amount of encapsulation efficiency as caffeine at low concentration. This might relate to attraction between opposite charge of NLC and ascorbic acid. When the active ingredients loaded NLC was subjected to in vitro release, the active ingredients’ release profile suggested that NLC exhibit sustained release properties whereby the rate of release is concentration dependent.

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Pardeike J, Hommoss A, Müller RH. Lipid nanoparticle (SLN, NLC) in cosmetic and pharmaceutical dermal products. Int J Pharm 2009; 366(1): 170-184.

Müller RH, Mehnert W, Lucks JS, et al. Solid lipid nanoparticles (SLN): An alternative colloidal carrier system for controlled drug delivery. Eur J Pharm Biopharm 1995; 41(1): 62-69.

Müller RH, Petersen RD, Hommoss A, et al. Nanostructured lipid carriers (NLC) in cosmetic dermal products. Adv Drug Deliv Rev 2007; 59(6): 522-530.

Woo JO, Misran M, Lee PF, et al. Development of a Controlled Release of Salicylic Acid Loaded Stearic Acid-Oleic Acid Nanoparticles in Cream for Topical Delivery. Sci World J 2014; 205703.

Obeidat WM, Schwabe K, Müller RH, et al. Preservation of nanostructured lipid carriers (NLC). Eur J Pharm Biopharm 2010; 76(1): 56-67.

Pople PV, Singh KK. Development and evaluation of colloidal modified nanolipid carrier: Application to topical delivery of tarcolimus. Eur J Pharm Biopharm 2011; 79(1): 82-94.

Souto EB, Müller RH. Cosmetic features and applications of lipid nanoparticles (SLN®, NLC®). International Journal of Cosmetic Science 2008; 30(3): 157-165.

Pardeike J, Schwabe K, Müller RH. Influence of nanostructured lipid carriers (NLC) on the physical properties of the Cutanova Nanorepair Q10 cream and the in vivo skin hydration effect. Int J Pharm 2010; 396(1): 166-173.

Sangsen Y, Laochai P, Chotsathidchai P, et al. Effect of solid lipid and liquid oil ratios on properties of nanostructured lipid carriers for oral curcumin delivery. Adv Mater Res 2015; 1060: 62-65.

Shoaib MH, Tazeen J, Merchant HA, et al. Evaluation of Drug Release Kinetics from Ibuprofen Matrix Tablets using HPMC. Pak J Pharm Sci 2006; 19(2): 119-124.

Dash S, Murthy PN, Nath L, et al. (2010) Kinetic Modeling On Drug Release From Controlled Drug Delivery Systems. Acta Pol Pharm – Drug Res 2010; 67(3): 217-223.


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