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Whole genome sequence of Streptomyces humi strain MUSC 119T isolated from intertidal soil

Hooi-Leng Ser, Wen-Si Tan, Wai-Fong Yin, Kok-Gan Chan, Nurul Syakima Ab Mutalib, Learn-Han Lee Abstract - 167 PDF - 118


Over the past few decades, microorganisms have made major contribution in natural product research, particularly those from the genus Streptomyces. Streptomyces humi MUSC 119T was previously isolated as novel streptomycete from mangrove soil in Malaysia. During the screening programme for bioactive strains, this strain was discovered to possess antioxidant activity – scavenging and reducing accumulation of free radicals in biochemical assays. Consequently, whole genome sequencing was performed to evaluate genomic potential of the strain. Based on our analysis, the genome size of MUSC 119T is described to be 10.01 Mbps with G + C content of 71.80%. Based on antiSMASH analysis, the strain possess great genomic potential, having nine biosynthetic gene clusters displaying high similarities to known gene clusters. These findings indicates that mangrove Streptomyces species like MUSC 119T may potentially play an important role in drug development process, while the availability of its whole genome sequences allows further manipulation to isolate and identify compound of interest.

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Bérdy J. Thoughts and facts about antibiotics: Where we are now and where we are heading. J Antibiotics 2012; 65(8): 385.

Subramani R, Aalbersberg W. Marine actinomycetes: An ongoing source of novel bioactive metabolites. Microbiol Res 2012; 167(10): 571-580.

Ser HL, Tan WS, Ab Mutalib NS, et al. Genome sequence of Streptomyces pluripotens MUSC 135T exhibiting antibacterial and antioxidant activity. Mar Gen 2015; 24: 281-283.

Sangkanu S, Rukachaisirikul V, Suriyachadkun C, et al. Evaluation of antibacterial potential of mangrove sediment-derived actinomycetes. Microbial Pathogen 2017; 112: 303-312.

Law JW, Ser HL, Khan TM, et al. The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Front Microbiol 2017; 8: 3.

Dhakal D, Pokhrel AR, Shrestha B, et al. Marine rare actinobacteria: Isolation, characterization, and strategies for harnessing bioactive compounds. Front Microbiol 2017; 8: 1106.

Ser HL, Tan LT, Law JW, et al. Focused review: Cytotoxic and antioxidant potentials of mangrove-derived Streptomyces. Front Microbiol 2017; 8: 2065.

Jose PA, Jha B. Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential. Sci Rep 2017; 7(1): 10041.

Qin S, Li WJ, Dastager SG, et al. Actinobacteria in special and extreme habitats: Diversity, function roles, and environmental adaptations. Front Microbiol 2016; 7: 1415.

Ser HL, Law JW, Chaiyakunapruk N, et al. Fermentation conditions that affect clavulanic acid production in Streptomyces clavuligerus: A systematic review. Front Microbiol 2016; 7: 522.

Sarmiento-Vizcaíno A, Braña AF, González V, et al. Atmospheric dispersal of bioactive Streptomyces albidoflavus strains among terrestrial and marine environments. Microbial Ecol 2016; 71(2): 375-386.

Tan LT, Chan KG, Chan CK, et al. Antioxidative potential of a Streptomyces sp. MUM292 isolated from mangrove soil. BioMed Res Int 2018; 2018.

Tan LT, Chan KG, Pusparajah P, et al. Mangrove derived Streptomyces sp. MUM265 as a potential source of antioxidant and anticolon-cancer agents. BMC Microbiol 2019; 19(1): 38.

Ser HL, Yin WF, Chan KG, et al. Antioxidant and cytotoxic potentials of Streptomyces gilvigriseus MUSC 26T isolated from mangrove soil in Malaysia. Prog Microbes Mol Biol 2018; 1(1).

Ghosh S, Kuisiene N, Cheeptham N. The cave microbiome as a source for drug discovery: Reality or pipe dream?. Biochem Pharm 2017; 134: 18-34.

Law JW, Ser HL, Ab Mutalib NS, et al. Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. Sci Rep 2019; 9(1): 3056.

Li K, Tang X, Zhao J, et al. Streptomyces cadmiisoli sp. nov., a novel actinomycete isolated from cadmium-contaminated soil. Int J Syst Evol Microbiol 2019.

Adam D, Maciejewska M, Naômé A, et al. Isolation, characterization, and antibacterial activity of hard-to-culture actinobacteria from cave moonmilk deposits. Antibiotics 2018; 7(2): 28.

Zainal N, Ser HL, Yin WF, et al. Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest. Antonie van Leeuwenhoek 2016; 109(3):467-74.

Law JW, Tan KX, Wong SH, Ab Mutalib NS, Lee LH. Taxonomic and characterization methods of Streptomyces: a review. Prog Microbes Mol Biol 2018; 1(1).

Ser HL, Tan WS, Cheng HJ, et al. Draft genome of amylolytic actinobacterium, Sinomonas humi MUSC 117T isolated from intertidal soil. Mar Gen 2015; 24: 209-210.

Ser HL, Ab Mutalib NS, Yin WF, et al. Genome sequence of Streptomyces antioxidans MUSC 164T isolated from mangrove forest. Prog Microbes Mol Biol 2018. url:

Ser HL, Tan WS, Cheng HJ, et al. Draft genome of starch-degrading actinobacterium, Microbacterium mangrovi MUSC 115T isolated from intertidal sediments. Prog Drug Dis Biomed Sci 2018. url:

Ser HL, Tan WS, Ab Mutalib NS, et al. Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments. Braz J Microbiol 2018; 49(1): 13-15.

Aziz RK, Bartels D, Best AA, et al. The RAST Server: Rapid annotations using subsystems technology. BMC Genomics 2008; 9: 75.

Lowe TM, Eddy SR. tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence. Nuc Acids Res 1997; 25: 955-964.

Lagesen K, Hallin P, Rodland EA, et al. RNAmmer: Consistent and rapid annotation of ribosomal RNA genes. Nuc Acids Res 2007; 35: 3100-3108.

Hyatt D, Chen GL, Locascio PF, et al. Prodigal: Prokaryotic gene recognition and translation initiation site identification. BMC Bioinform 2010; 11: 119.


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