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A reliable and affordable 3D tumor spheroid model for natural product drug discovery: A case study of curcumin

Loh Teng Hern Tan, Liang Ee Low, Siah Ying Tang, Wei Hsum Yap, Lay Hong Chuah, Chim Kei Chan, Learn Han Lee, Bey Hing Goh Abstract - 917 PDF - 649

Abstract


Three-dimensional cell culture methods revolutionize the field of anticancer drug discovery, forming an important link-bridge between conventional in vitro and in vivo models and conferring significant clinical and biological relevant data. The current work presents an affordable yet reproducible method of generating homogenous 3D tumor spheroids. Also, a new open source software is adapted to perform an automated image analysis of 3D tumor spheroids and subsequently generate a list of morphological parameters of which could be utilized to determine the response of these spheroids toward treatments. Our data showed that this work could serve as a reliable 3D cell culture platform for preclinical cytotoxicity testing of natural products prior to the expensive and time-consuming animal models


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